Protein Technologies Unit
Protein Technologies Unit
Protein Technologies Unit
Overview
The Protein Technologies Unit provides the researchers at the CRG and PRBB, external institutions and private companies with a new, high quality technology platform to perform protein production and automated processes in the context of biomolecular and biochemical screening assays.
Through staff-assisted screening, users will have access to the equipment of the facility including liquid handlers, plate readers and the small molecule libraries.
In addition, the unit is aimed at supporting the researchers of the CRG institute by purchasing, seeking and maintaining valuable stocks of clones and strains.
Contact: bmspt@crg.eu
You can learn more about the unit by taking the virtual tour HERE.
Related Links
Protein Production and Purification Partnership in Europe
Association of Resources for Biophysical Research in Europe
Protein Quality Guidelines for Biophysical and Biochemical Studies
Carlo Carolis CV
Since July 2013: Head of the Protein Technologies Facility, CRG, Barcelona, Spain
2010-2013: Senior Technical Officer, Biomolecular Screening & Protein Technologies Facility, CRG, Barcelona, Spain
2008-2010: Postdoctoral researcher, Structural and Computational Biology Programme, IRB, Barcelona, Spain
2008: Postdoctoral researcher, EMBL, Heidelberg, Germany
2007: PhD in Structural Biology, EMBL, Heidelberg, Germany
How to access
All services offered by the Protein Technologies Unit are accessible to all the scientific community. Access is always open and services are performed upon request.
All services and equipment offered by the Protein Technologies Unit are accessible by the web platform AGENDO.
You can access to AGENDO in the following link: https://crg.agendoscience.com/
Service Prices
Services
- Full-service production of plasmid clones, mutants, and plasmid libraries.
- High-throughput cloning
- Production of recombinant proteins and protein complexes: bacteria, insect cells and mammalian expression
- Protein and antibody purification
- Biophysical characterization of proteins and nucleic acids:
- Surface plasmon resonance, Microscale thermophoresis, Size Exclusion Chromatography - Multi Angle Light Scattering
- Automation of biomolecular and biochemical screening assays
- Genome editing using the Crispr-Cas9 system: Gene knockout/Knockin cell line engineering in collaboration with the Tissue Engineering Unit at the CRG
Equipment
- ÄKTA Xpress twin and ÄktaFPLC system for protein purification (GE Healthcare)
- Biacore T100 (SPR)
- Monolith NT.115 Thermophoresis (MST)
- UPLC (shimatzu)- DAWN8+ Optilab® T-rEX™
- LabCHIP GXII
- Robotic Platform TECAN Freedom evo 200
- Robotic Platform Caliper Zephyr
- Robotic Platform Caliper Sciclone
- Biotek ELx405 washer
- Kendro Incubator
- MultidropV2
- Tecan plate reader M200
- Amaxa electroporator 96 well plate
siRNA libraries
The human whole-genome collection siGENOME SMARTPoool, (Dharmacon, Thermo-Fisher) is composed of pools of 4 different siRNAs per gene and is distributed in four "sets" as follows
- Kinases - 779 genes
- GPCR - 516 genes
- ‘Druggable’ genome - 6022 genes
- Remaining Genome collection – 13803 genes
Chemical compound libraries
- The Prestwick Chemical Library® - 1200 small molecules, 100% being marketed drugs
- Prestwick Phytochemical Library - 320 natural products
- Tocris kinase inhibitors toolbox (Tocris) – 80 small molecule compounds
Other Resources for CRG Users Only (you will need to log in into the CRG Intranet)
Head of unit
Technicians
Technicians
